Figure 8.

Characterization of resident peritoneal cells and thioglycollate-elicited peritoneal macrophages. (a) Southern blot analysis of deletion in FACS-purified resident peritoneal CD11b^high cells from mice with the indicated genotypes and (b) in thioglycollate-elicited IKK2^fl and IKK2^del peritoneal macrophages. Indicated are the presence or absence (+ or –) of Cre-recombinase in the macrophages, the WT, floxed (fl), and deleted (d) allele, and the percentage of deletion calculated by quantification of the ratio between the floxed and deleted band. IKK2^fl (c) and IKK2^del (d) thioglycollate-elicited macrophages were untreated (white) or stimulated with LPS (gray). TNF production was detected by intracellular cytokine staining and analyzed by FACS. (e) IKK2^fl and IKK2^del macrophages were stimulated with LPS, and TNF production was detected by intracellular cytokine staining and analyzed by FACS. Macrophages were identified as F4/80⁺. Results are representative for at least two experiments. Error bars indicate SEM. *P < 0.01.