Figure 5.

Effect of QS variants of p53 on cell viability and apoptosis. Apoptosis and cell viability were assessed 48 hours following infection with either control adenovirus or adenoviruses expressing the indicated variants of p53. Apoptosis was assessed by subdiploid DNA content (A) and viability was assessed by Trypan blue exclusion (B). Each point represents the mean (±SEM) determined from three independent experiments. Adp53wt induced more apoptosis than the variants (one-way analysis of variance followed by Tukey's Multiple Comparisons test, P ≤ .01 for apoptosis and P ≤ .05 for viability). No significant difference in viability or apoptosis was observed when comparing QS1 and QS2 variants.