Figure 3.

Time-lapse of mAb 806 internalization in A431 cells expressing dynamin K44A-GFP. (A) Cells were transiently transfected with DynK44A-GFP (red asterisk). Panels representing images at 5, 10, 20, 30, and 60 minutes after mAb 806-Cy3 internalization are shown. (B) Mean ratio of intracellular to membrane (black) and membrane to intracellular (red) fluorescence of untransfected wt cells ± SEM (n = 4), over time. Untransfected cells show gradual increase in intracellular fluorescence with a concurrent decrease in membrane associated fluorescence. (C) Mean ratio of intracellular to membrane (black) and membrane to intracellular (red) fluorescence of DynK44A-transfected cells ± SEM (n = 6), over time. DynK44A-transfected cells show little accumulation of intracellular fluorescence while sustaining high membrane fluorescence intensity. Scale bar, 20 µm. Refer to Supplemental data for Video W2.