Figure 2.
Arabidopsis TAN::YFP localizes as a ring at the division site throughout mitosis and cytokinesis in root tip cells. A-P, Dual localization of 35S-AtTAN::YFP (monochrome in second column, green in third and fourth column) and 35S-CFP::TUA1 (monochrome in first column, red in third and fourth columns) in cells at the indicated cell cycle stages. Brackets in A indicate a pair of adjacent PPBs. Arrowheads point to a metaphase spindle in E, and to phragmoplasts in I and M. Arrow in G points to the junction between a spindle-associated AtTAN::YFP ring and the adjacent PPB. 45° rotations in D, H, and L show that AtTAN::YFP forms a complete ring encircling the cell. Scale bar = 10 μm.