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. Author manuscript; available in PMC: 2008 Apr 30.
Published in final edited form as: Cell Immunol. 2007 Sep 7;247(1):36–48. doi: 10.1016/j.cellimm.2007.07.004

Figure 5.

Figure 5

HIV peptides induce GzB secretion in PBMC of HIV-infected donors. GzB and IFN-γ secretion by CD8+ cells is dissociated in these donors. (a and b), PBMC were obtained from 7 HIV-infected individuals and tested in 24 h ex vivo IFN-γ (a) and GzB (b) ELISPOT assays. A peptide library covering HIV-1 gag peptides 1 – 480 was used for recall. The peptides were 20-mers and walked the sequence of gag in steps of 5 a.a. They were tested individually (as specified by the peptide serial numbers on the x-axis), in duplicate well. The means of the duplicates are shown. (c and d) Because 20 a.a. long peptides can induce CD4+ cells in addition to CD8+ cells, PBMC of HIV-infected subjects were CD4 cell-depleted before testing with the HIV peptide library in IFN-γ (c) and GzB (d) ELISPOT assays. Since all HIV+ donors had known low CD4+ cell count (approx. 150 CD4+ cells/µl), similar numbers of CD8+ T cells were used for antigen stimulation in both the total (a and b) and the CD4+ depleted (c and d) cell populations. Selected peptides were tested that previously scored positive/negative. An asterisk highlights peptides that either induced IFN-γ or GzB only.