Figure 5. Loss of p27 Rescues the lck ^−/− Phenotype.

(A) Representative FACS analysis of thymi from lck ^−/− p27^+/+, lck ^−/− p27 ^+/−, and lck ^−/− p27 ^−/− mice indicating loss of one copy of the p27 gene rescues the lck ^−/− phenotype.

(B) The total cellularity for thymi and DP cells from all three genotypes are shown in the graph. Data were collected from three lck ^−/− p27^+/+, seven lck ^−/− p27 ^+/−, and seven lck ^−/− p27 ^−/− mice.

(C) Quantitative RT-PCR detected transcript levels for Id3 and p27 in wild-type small DN3 and DN4 cells. The fold change in the mRNA levels in the DN4 cells is relative to the DN3 cells.

doi:10.1371/journal.pgen.0030219.g005