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. 2007 Dec 7;3(12):e174. doi: 10.1371/journal.ppat.0030174

Figure 1. Genomic Localization and Protein Organization of γHV-68 vMAP.

Figure 1

(A) vMAP is encoded within the ORF57. (Top diagram) The vMAP genomic localization is shown within the ORF57 sequence. Numbers indicate the nucleotide position according to Entrez accession number U97553. The grid box represents a 90–base pair intron of ORF57 and the box with vertical lines represents vMAP (M8). (Bottom diagram, sequence) The first ATG denotes the start codon for ORF57 (arrow on the left). The coding sequence of vMAP is shown in reference to ORF57 and its amino acid sequence is listed below. The translation of vMAP has a +1 shift to ORF57 frame; thus, the vMAP amino acid sequence is completely different from the ORF57 amino acid sequence. The mitochondrion-targeting sequence (MTS) is italicized and the putative transmembrane (TM) domain is indicated by bold letters. An arrow (↓) over a gap (dots) indicates the splicing site where a 90–base pair of intron is removed [47]. *, stop codon.

(B) vMAP protein organization. The N terminal sequence (aa 1–49) serves as a mitochondrial targeting sequence (MTS) as well as a Bcl-2-interacting region. Both the internal hydrophobic region (aa 50–66) and the putative transmembrane (TM) domain (aa 135–157) interact with VDAC1.