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. 2007 Dec 7;3(12):e201. doi: 10.1371/journal.ppat.0030201

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© 2007 Tsetsarkin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Ae. albopictus (A) and Ae. aegypti (C) mosquitoes were orally infected with LR-GFP-226V and LR-GFP-226A. At the indicated time points, 16–21 mosquitoes were dissected and salivary glands were analyzed for eGFP expression. Percent of dissemination was estimated as a ratio of the number of mosquitoes with eGFP-positive salivary glands to the number of mosquitoes with eGFP-positive midguts. For Ae. albopictus, infectious blood meal titers were 5.95 and 6.52 Log₁₀TCID₅₀/ml for LR-GFP-226V and LR-GFP-226A, respectively. For Ae. aegypti, the infectious blood meal titer was 6.95 Log₁₀TCID₅₀/ml for both LR-GFP-226V and LR-GFP-226A viruses. Dissemination rates were compared statistically by Fisher's exact test using SPSS version 11.5. Asterisk indicates p < 0.05.

(B and D) Competition between LR-ApaI-226V and LR-226A for dissemination into heads of Ae. albopictus and Ae. aegypti mosquitoes. 10⁷ pfu of LR-ApaI-226V and LR-226A were mixed and orally presented to Ae. albopictus (B) and Ae. aegypti (D). Viral RNAs were extracted from four pools of five heads collected at 12 dpi. RT-PCR products were digested with ApaI, separated in 2% agarose gel, and gels were stained using ethidium bromide.

BM - initial ratio of LR-ApaI-226V and LR-226A in blood meal samples. 1–4 ratio of LR-ApaI-226V and LR-226A RNA in four independent replicas of the five pooled heads per replica.