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. 2007 Dec 7;3(12):e201. doi: 10.1371/journal.ppat.0030201

Figure 4. Effect of E1-A226V Mutation on CHIKV Kinetics of Viral Growth in Bodies of Ae. albopictus Mosquitoes.

Figure 4

(A) Virus production in orally infected Ae. albopictus mosquitoes. Infected mosquitoes were sampled at 0, 1, 2, 3, 5, 7, and 14 dpi and titrated on Vero cells to estimate average titer ± standard deviation of eight whole mosquitoes. Differences in viral titers were analyzed by pairwise t-tests. Asterisk indicates p < 0.05.

(B) Kinetics of competition between LR-ApaI-226V and LR-226A in bodies of Ae. albopictus mosquitoes. 107 pfu of LR-ApaI-226V and LR-226A were mixed and orally presented to Ae. albopictus. Infected mosquitoes were sampled at 1, 3, 5, 7, and 14 dpi. For each time point, viral RNA was extracted from two pools of ten mosquitoes.

BM - initial ratio of LR-ApaI-226V and LR-226A in blood meal samples.

RF - relative fitness of LR-Apa-226V to LR-226A was calculated as a ratio between 226V and 226A bands in the sample, divided to the control ratio between 226V and 226A in the blood meal. Results expressed as average of two replicas ± standard deviation.