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. 2007 Dec 7;3(12):e189. doi: 10.1371/journal.ppat.0030189

Figure 1. Identification of Lipoylated Proteins in P. falciparum .

Figure 1

P. falciparum protein extracts (15 μg) were separated on an SDS-PAGE (4%–12%, Invitrogen) and blotted onto nitrocellulose as described in the Materials and Methods section. The blots were analysed using four different antibodies: (lane 1) rabbit anti-H-protein at 1:2,000 recognising a 25 kDa band; (lane 2) rabbit anti-BCDH-E2 at 1:100 recognising a 50 kDa band; (lane 3) rat anti-KGDH-E2 at 1:5,000 recognising a 47 kDa band; (lane 4) rabbit anti-lipoic acid at 1:500 recognising four major protein bands corresponding to PDH-E2 (75 kDa, Allary et al. [14]), BCDH-E2 (50 kDa), KGDH-E2 (47 kDa) and H-protein (25 kDa).