Figure 3. SidG Contains a C-Terminal Translocation Signal.
(A) Schematic representation of the 965-aa SidG protein, which contains an extensive coiled-coil region (grey) as well as two hydrophobic segments (black). The identified translocation signal is depicted (T).
(B and C) CHO FcγRII cells were infected with wild-type (black) or ΔdotA (dark grey) strains expressing the indicated Cya-SidG hybrid proteins for 1 h. Total cAMP production induced by translocation of the hybrid was determined as in Figure 1.