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. Author manuscript; available in PMC: 2008 Jan 15.
Published in final edited form as: Free Radic Biol Med. 2007 Jul 20;43(10):1394–1408. doi: 10.1016/j.freeradbiomed.2007.07.011

Fig-5. Increased induction of key differentiation genes in DPSCs by NAC but not by Vitamin E or C at different stages of differentiation.

Fig-5

Fig-5

Fig-5

(A) Rat DPSCs (5×105/ml) were cultured in media alone (undifferentiated) or with the combination of β-glycerophosphate (10 mM) and ascorbic acid (50μg/ml) (Differentiated), or with the combination of β-glycerophosphate (10 mM), ascorbic acid (50μg/ml) and Dexamethsone (10−8M) (Differentiated+Dex) for 14 days after which the cells were treated with NAC (20 mM) overnight and the levels of Osteopontin, Osteocalcin and Dentin Sialoprotein gene induction were determined by RT-PCR. GAPDH was used as the loading control. One of three independent experiments is shown in this figure (B) Rat DPSCs (5×105/ml) cultured with the combination of β-glycerophosphate (10 mM) and ascorbic acid (50μg/ml) were treated with the varying concentrations of HEMA as indicated in the figure and NAC (20 mM) for 18 hours. Total RNA was then extracted, and the levels of ostepontin (OPN) were determined. GAPDH was used as the loading control. One of three independent experiments is shown in this figure. (C) Human DPSCs (5×105/ml) cultured with the combination of β-glycerophosphate (10 mM) and ascorbic acid (50μg/ml) were treated with HEMA as indicated in the figure and NAC (20 mM) for 18 hours. Total RNA was then extracted, and the levels of ostepontin (OPN) were determined. GAPDH was used as the loading control. One of three independent experiments is shown in this figure. (D) Rat DPSCs (5×105/ml) cultured with the combination of β-glycerophosphate (10 mM) and ascorbic acid (50μg/ml) were left untreated or treated with HEMA (0.0082M), NAC (20 mM), or Vitamin E (5 mM), or their combination as indicated in the figure for 18 hours. Total RNA was then extracted, and the levels of ostepontin (OPN) were determined. GAPDH was used as the loading control. One of three independent experiments is shown in this figure. (E) Rat DPSCs (5×105/ml) cultured in DMEM culture medium were either left untreated or treated with HEMA (0.0082M), NAC (20 mM), or Vitamin C (5 μg/ml), as indicated in the figure for 18 hours. Total RNA was then extracted, and the levels of ostepontin (OPN) were determined. GAPDH was used as the loading control. One of three independent experiments is shown in this figure.