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. Author manuscript; available in PMC: 2008 Sep 7.
Published in final edited form as: Cell. 2007 Sep 7;130(5):918–931. doi: 10.1016/j.cell.2007.07.009

Figure 5. Increased IL-1β release is independent of apoptosis in IKKβ-deficient neutrophils.

Figure 5

(A) Levels of IL-1β and TNF-α mRNAs in neutrophils after LPS stimulation were examined by RNase protection. (B) Immunoblot analysis of intracellular pro-IL-1β and pro-TNF-α in neutrophils after LPS stimulation. (C, D) IL-1β and TNF-α secretion by LPS-stimulated neutrophils determined by ELISA. (E) Number of annexin V positive Gr-1+ cells determined by flow cytometry. (F) Immunoblot analysis of cleaved caspase-3 in neutrophils after LPS stimulation (100 ng/ml). (G) IL-1β secretion by LPS-stimulated wt and caspase-1−/− macrophages and neutrophils. (H) Bioassay of supernatants of LPS-stimulated wt and caspase-1−/− neutrophils using EL4 cells.