Figure 3.
Effect of TSA on growth and DNA methylation. (A) Liquid cultures inoculated with N. crassa strain N644 (7 × 104 conidia per ml) were supplemented with up to 1 μg/ml (3.3 μM) TSA and grown 27 hr. The untreated culture yielded 32.8 mg of dry tissue. (B) Southern hybridization of selected samples from the cultures. Samples of DNA were prepared and digested with DpnII (D) or Sau3AI (S) and probed for am sequences. Stronger signals in the 0.33 μM lanes is partially due to 2× heavier loadings of DNA in these lanes. The positions of selected size standards (kb) are indicated.