Figure 2.

Northern blot of total RNA isolated from wild-type and p^6H/p^6H tissues (B, brain; L, liver; and T, testis) and hybridized with BS2c probe (corresponding to nucleotides 12661–14606 of the final rjs cDNA contig, which includes part of RLDc and all of the HECT domain). Markers (not shown) indicate this to be a very large transcript, ≥15 kb. Note the expression of the rjs gene in brain and testis of wild type and the absence of transcript in p^6H/p^6H total RNA. The faint high molecular weight band in p^6H/p^6H brain total RNA probably represents nonspecific hybridization since RT-PCR from p^6H/p^6H mRNA was negative with primers after nucleotide 1993.