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. 1998 Aug 4;95(16):9442–9447. doi: 10.1073/pnas.95.16.9442

Figure 3.

Figure 3

Deficient activation of NFκB and IRF-1 by dsRNA in P2.1 cells. (A) Activation of NFκB for binding to a PRDII element. EMSAs were performed with whole cell extracts prepared from U4C and P2.1 cells, untreated or treated for 3 hr with 40 ng/ml of TNFα or 100 μg/ml of dsRNA. (B) Activation of IRF-1 for binding to an ISG15 ISRE. EMSAs were performed with whole cell extracts from U4C and P2.1 cells, untreated or treated with 100 μg/ml of dsRNA for the times shown above each lane. (C) Western analysis for IRF-1 protein levels in the extracts used for the EMSA experiments. The sizes of molecular weight standards are indicated in kilodaltons.