Abstract
Calcium transport was investigated in membrane vesicles prepared from the oral bacterium Streptococcus sanguis. Procedures were devised for the preparation of membrane vesicles capable of accumulating 45Ca2+. Uptake was ATP dependent and did not require a proton motive force. Calcium transport in these vesicles was compared with 45Ca2+ accumulation in membrane vesicles from Streptococcus faecalis and Escherichia coli. The data support the existence of an ATP-driven calcium pump in S. sanguis similar to that in S. faecalis. This pump, which catalyzes uptake into membrane vesicles, would be responsible for extrusion of calcium from intact cells.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ambudkar S. V., Zlotnick G. W., Rosen B. P. Calcium efflux from Escherichia coli. Evidence for two systems. J Biol Chem. 1984 May 25;259(10):6142–6146. [PubMed] [Google Scholar]
- Bürkler J., Solioz M. The ATP-dependent Ca2+-pumping system of Streptococcus faecium. Ann N Y Acad Sci. 1982;402:422–432. doi: 10.1111/j.1749-6632.1982.tb25758.x. [DOI] [PubMed] [Google Scholar]
- Fabiato A., Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol (Paris) 1979;75(5):463–505. [PubMed] [Google Scholar]
- Harold F. M., Pavlasová E., Baarda J. R. A transmembrane pH gradient in Streptococcus faecalis: origin, and dissipation by proton conductors and N,N'-dicyclohexylcarbodimide. Biochim Biophys Acta. 1970;196(2):235–244. doi: 10.1016/0005-2736(70)90011-8. [DOI] [PubMed] [Google Scholar]
- Kobayashi H., Van Brunt J., Harold F. M. ATP-linked calcium transport in cells and membrane vesicles of Streptococcus faecalis. J Biol Chem. 1978 Apr 10;253(7):2085–2092. [PubMed] [Google Scholar]
- Macrina F. L., Wood P. H., Jones K. R. Simple method for demonstrating small plasmid deoxyribonucleic acid molecules in oral streptococci. Appl Environ Microbiol. 1980 May;39(5):1070–1073. doi: 10.1128/aem.39.5.1070-1073.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]