Figure 4.

Pre-Tα transcripts are expressed in CD3⁻CD8αα⁺CD16⁺ IEL from mice exhibiting a block in T cell development. RNA was extracted from sorted CD3⁻CD8αα⁺CD16⁺ and CD3⁻CD8α⁻ IEL isolated from pools of six 5- to 10-week-old mice of the indicated genotypes. Thymocytes from the same animals and splenocytes from wild-type C57BL/6 mice served as positive and negative controls, respectively. RT-PCR and detection of the amplification products were performed as for Fig. 3.