Abstract
Sera of fourteen rabbits injected with alum-precipitated recrystallized ovaltumin, containing 0.046 to 0.604 mg. of precipitable antibody nitrogen per ml. (average 0.299 mg.), passively sensitized human skin, while the sera of nine rabbits injected with dissolved recrystallized ovalbumin, containing from less than 0.05 to 0.420 of antibody nitrogen per ml. (average 0.176 mg. or less), were inactive in human skin. The skin-sensitizing activity of the sera bore no relation to the precipitin content. Removal of 68 to 90 per cent of the precipitin nitrogen by a single addition of antigen did not affect the activity of the sera in sensitizing human skin. Removal of all precipitable antibody nitrogen in one serum by a single addition of antigen removed the skin-sensitizing activity. The "univalent" antibody remaining after complete removal of precipitin by fractional addition of antigen showed the same activity in passive sensitization of human skin as the original serum.
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Selected References
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- BENACERRAF B., KABAT E. A. A quantitative study of the Arthus phenomenon induced passively in the guinea pig. J Immunol. 1950 Jan;64(1):1–19. [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE STUDY OF THE PRECIPITIN REACTION BETWEEN TYPE III PNEUMOCOCCUS POLYSACCHARIDE AND PURIFIED HOMOLOGOUS ANTIBODY. J Exp Med. 1929 Nov 30;50(6):809–823. doi: 10.1084/jem.50.6.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : III. THE REACTION BETWEEN CRYSTALLINE EGG ALBUMIN AND ITS HOMOLOGOUS ANTIBODY. J Exp Med. 1935 Oct 31;62(5):697–720. doi: 10.1084/jem.62.5.697. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Kendall F. E., Soo Hoo C. M. QUANTITATIVE STUDIES ON THE PRECIPITIN REACTION : ANTIBODY PRODUCTION IN RABBITS INJECTED WITH AN AZO PROTEIN. J Exp Med. 1933 Jul 31;58(2):137–152. doi: 10.1084/jem.58.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heidelberger M., Treffers H. P., Mayer M. A QUANTITATIVE THEORY OF THE PRECIPITIN REACTION : VII. THE EGG ALBUMIN-ANTIBODY REACTION IN ANTISERA FROM THE RABBIT AND HORSE. J Exp Med. 1940 Jan 31;71(2):271–282. doi: 10.1084/jem.71.2.271. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KABAT E. A., BENACERRAF B. A quantitative study of passive anaphylaxis in the guinea pig; passive sensitization with non-precipitable or univalent rabbit antiovalbumin. J Immunol. 1949 May;62(1):97–101. [PubMed] [Google Scholar]
- Kendall F. E. STUDIES ON SERUM PROTEINS. I. IDENTIFICATION OF A SINGLE SERUM GLOBULIN BY IMMUNOLOGICAL MEANS. ITS DISTRIBUTION IN THE SERA OF NORMAL INDIVIDUALS AND OF PATIENTS WITH CIRRHOSIS OF THE LIVER AND WITH CHRONIC GLOMERULONEPHRITIS. J Clin Invest. 1937 Nov;16(6):921–931. doi: 10.1172/JCI100918. [DOI] [PMC free article] [PubMed] [Google Scholar]