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. Author manuscript; available in PMC: 2007 Dec 13.
Published in final edited form as: Am J Physiol Lung Cell Mol Physiol. 2006 Jun 9;291(5):L957–L965. doi: 10.1152/ajplung.00065.2006

Fig. 7.

Fig. 7

1-EBIO-evoked membrane currents in KCNN4-expressing CHO cells. A: relationships between VHold and I derived from studies of KCNN4-transfected cells undertaken with a pipette filing solution designed to hold [Ca2+]i at 0.05 μM. Data (means ± SE; n = 5) were obtained during superfusion with control saline and after 1- to 2-min exposure to 1 mM 1-EBIO. B: data from directly analogous experiments (n = 6) undertaken with a pipette filling solution designed to hold [Ca2+]i at 0.2 μM. C: pooled data showing the effects of [Ca2+]i and 1-EBIO on I30mV. Statistically significant effects of 1-EBIO (Student’s paired t-test): ***P < 0.005. D: continuous records (means ± SE) showing the effects of 1-EBIO on the current required to hold Vm at -40 mV (I-40mV) with pipette filling solutions designed to hold [Ca2+]i at 0.2 (n = 4) or 0.05 (n = 5) μM. This potential was chosen as it equates to the equilibrium potential for Cl- (ECl), implying that K+ is the only ion able to carry outward current under the present conditions.