Table 1.

Effects of lowering [Cl^-]_o on V_m

		Vm, mV
Replacement Anion	n	Control	Low Cl-	ΔVm, mV
Gluconate	17	-40.6±3.4	-30.9±4.8‡	9.7±2.5
Methanesulfonate	7	-53.3±5.5	-37.5±4.5*	15.8±5.2
I	7	-33.5±6.9	-44.1±9.1†	-10.6±2.8

Data are means ± SE for n cells in each group. Values of membrane potential (V_m) measured under zero current clamp from amiloride-treated (10 μM) cells superfused successively with control saline [Cl^- concentration ([Cl^-]) = 151.5 mM] and with saline in which extracellular [Cl^-] ([Cl^-]_o) had been lowered to 29 mM by replacing this anion with gluconate, methanesulfonate, or I. Shifts in Vm (ΔV_m) were quantified by subtracting the value measured at low [Cl^-]_o from that measured in the same cells under control conditions. Significant effects of low Cl^- (Student’s paired t-test):

^*P < 0.05

^†P < 0.005

^‡P < 0.002