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. 1997 Jun 2;137(5):1137–1147. doi: 10.1083/jcb.137.5.1137

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Change in CD44 epitope expression during in vitro cultivation of epidermal LC and blood DC. (A) Human LC freshly isolated and after 48 and 72 h of culture and (C) mouse DC generated from C57/BL6 bone marrow were stained with mAbs against HLA-DR or Ia^b, 7-aminoactinomycin-D, and mAbs directed against CD44 epitopes. Mean fluorescence intensity of viable and HLA-DR+ cells was determined by FACScan^® and is shown in the upper right corner of each graph. Data is representative for six independent experiments. Low expression of CD44v epitopes on freshly isolated LC was not caused by the trypsinization used during the isolation procedure, since identical trypsinization of cultured LC did not affect their CD44v expression significantly (not shown). (B) Corresponding analysis of DC generated from human peripheral blood monocytes gated for CD1a. Data is representative for four independent experiments.