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. 1997 Jun 2;137(5):1137–1147. doi: 10.1083/jcb.137.5.1137

Figure 3.

Figure 3

Figure 3

CD44 expression pattern on LC migrating from epidermis into dermis. Cryosections of skin cultured for 12 h were stained with Lag mAb followed by secondary FITC-conjugated F(ab), goat anti–mouse IgG (H+L) to detect LC (green), and CD44 mAbs followed by a Cy3-conjugated goat F(ab)2 anti–mouse IgG (H+L) antibody (red). Double-positive cells appear yellow-orange. (A) Staining with Lag and anti–pan CD44. Lag+ cells within the epidermis (1) and those that migrated into the dermis (2) stained double positive for Lag and pan CD44 (yellow). Keratinocytes express pan CD44 but not Lag (red). Lag+ intra-epidermal LC also express the epitope created by exons v7/v8 (B) but very low levels of CD44 exon v5 (C), v6 (E), and v9 (G) epitopes. Lag+ cells that migrated into dermis express CD44v5 (D), v6 (F), and v9 (H). (I and J) LC that had migrated from split thickness skin were gated for CD1a expression, stained with the mAbs indicated, and analyzed by FACS® as in Fig. 1. Bar, 10 μm.