Table IV.

Percent of LAMP-1/LAMP-2 Point Mutant Chimera Expressed at the Cell Surface

Chimera		Cell line		n*		Average surface expression (%)‡
L/2b/2b S396T		pool		2		40
		3		3		44 ± 9
		6		3		45 ± 9
L/2b/2b R391Y		4		2		40
		5		2		31
		6		2		28
		8		2		36
		14		2		24
		17		2		32
		19		2		29
		23		2		44
L/2b/2b V397L		pool		3		8 ± 2
		7		3		4 ± 1
		13		3		6 ± 1
L2b/2b S396T, V397L		pool		6		8 ± 2
		6		3		5 ± 1
		19		2		6
		21		2		6
		24		3		5 ± 2
L/2c/2c L396V		pool		5		22 ± 2
		3		2		21
		12		2		28

^*  n represents the number of independent experiments in which each sample was assayed in triplicate.  

^‡ average per cell line ± standard deviation; calculated from ¹²⁵I-labeled mAb-CV24 binding to intact and permeabilized cells as described in Materials and Methods.