Table II.
Distribution of the LET-2 Type IV Collagen Chain in let-2 Mutants*
| Strain | Percentage punctate intracellular stain‡ | Percentage pharyngeal basement membrane stain§ | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 15°C | 20°C | 25°C | 15°C | 20°C | 25°C | |||||||
| N2 | 3 | 0 | 10 | 100 | 87 | 87 | ||||||
| unc-3(e151) | 0 | 0 | 0 | 100 | 100 | 100 | ||||||
| let-2(g25) | ND | 15 | 73 | ND | 97 | 21 | ||||||
| let-2(b246) | 0 | 0 | 79 | 97 | 100 | 24 | ||||||
| let-2(mn139) | ND | 57 | 85 | ND | 55 | 9 | ||||||
| let-2(mn109) | 48 | 45 | 93 | 100 | 68 | 2 | ||||||
| let-2(g30) | 0 | 100 | 94 | 91 | 24 | 1 | ||||||
| let-2(mn129) | ND | 60 | 83 | ND | 83 | 33 | ||||||
*
Embryos from homozygous mutant or wild-type (N2) mothers were stained with LET-2 and UNC-54 antisera. Only embryos that showed UNC-54 stain were examined. At least 30 embryos were scored for each strain at each temperature.
‡
The percentage of twofold stage or later embryos that showed strong intracellular LET-2 stain in body wall muscle cells.
§
The percentage of twofold stage or later embryos that showed strong LET-2 stain of the pharyngeal basement membrane.