Figure 6.

Microinjection of wild-type CD4 and tailless CD4 into Hela-Lck cells. HeLa cells stably expressing human Lck were microinjected with cDNA for wildtype CD4 (left) or tailless CD4 (right). Cells were fixed 3 h after injection and stained with antibodies against Lck and CD4 (as in Fig. 1). Note the difference in distribution of Lck between cells that were injected with wild-type CD4 cDNA (left, the three cells on the left) and cells that were not injected. Also note that injection with tailless CD4 cDNA has no effect on the distribution of Lck (right, the cell in the middle). Confocal images of 3-μm optical sections are shown. Bar, 15 μm.