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. 1997 Jun 2;137(5):989–1000. doi: 10.1083/jcb.137.5.989

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Complete amino acid sequence of Nup84 derived from cDNA sequence analysis. The boxed segments correspond to the COOH-terminal predicted coiled–coil regions, which are most closely related to members of the tropomyosin family. The primary structure of the underlined peptides was initially elucidated by limited amino acid sequence analysis of Nup84 tryptic fragments. The highlighted peptide (P1), also obtained by amino acid sequence analysis, was used in the design of the oligonucleotide employed in the isolation of the cDNA. The GenBank accession number of the complete cDNA sequence is U93692.