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. 1997 Jun 2;137(5):989–1000. doi: 10.1083/jcb.137.5.989

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Double indirect immunofluorescence microscopy of BHKgrβ cells transiently expressing HA-CAN/Nup214. 24 h posttransfection and before fixation, the cells were treated with dexamethasone for periods up to 3 h as indicated at the top of the figure. The primary antibodies were 12CA5 (anti-HA, upper row) and a rabbit anti–β-galactosidase (corresponding fields, lower row). It is evident that there is a transient association of glucocorticoid receptor–β-galactosidase with the CAN/Nup214 foci shortly after the addition of dexamethasone (indicated with arrows in C and D). This is consistent with the observation that importins-α and -β are also associated with these structures (Fig. 6). Bar, 10 μm.