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. 1998 Aug 4;95(16):9500–9505. doi: 10.1073/pnas.95.16.9500

Figure 5.

Figure 5

Transactivation by Zf9 following transient transfection. (A) Cotransfection of 500 ng of either full length Zf9 (Zf9-GAL4) or the 201-aa N-terminal domain (ZAD-GAL4) fused to the GAL4 DNA binding domain and a GAL4 responsive luciferase reporter results in equally strong transactivation in Hep 3B cells. (B) Cotransfection of 100- to 1,000-ng Zf9 (pAC-Zf9) and a 1-μg luciferase reporter containing the SV40 promoter/enhancer (pGL Control) in D. schneider S2 cells demonstrates transactivation by full length Zf9 but not by either the N-terminal 201-aa (ZAD) or the 82-aa zinc finger domain (DNA binding domain). Data represents mean of two experiments normalized to cell number.