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. 1997 Sep 8;138(5):1149–1157. doi: 10.1083/jcb.138.5.1149

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ICAP-1α is a novel serine/threonine-rich 20-kD protein. (A) Sequence of ICAP-1. The translated amino acid sequence of ICAP-1α is shown. 50 amino acids absent in ICAP-1β are indicated in underlined italics. The nucleotide sequence data are available from GenBank/EMBL/DDBJ under accession number AF012023. (B) Northern blot analysis of ICAP-1α. Northern blots of transcripts from various human tissues (Clontech) were hybridized with ICAP-1α cDNA probe. The 0.9-kb mRNA is expressed in all eight tissue samples. The minor 1.3-kb mRNA, derived from the use of the downstream polyadenylation site, is also expressed in varying amount in all eight samples. (C) Western blot analysis of ICAP-1. ICAP-1α and ICAP-1β were detected in the total cell lysate (5 μg) from 293T cells using polyclonal anti-ICAP-1 antibody (lane 1). The assignments of ICAP-1α and ICAP-1β were confirmed by transiently expressing ICAP-1α (lane 2) and ICAP-1β cDNA (lane 3) in 293T cells. (D) The full-length 200-aa ICAP-1α and an alternatively spliced ICAP-1β lacking internal 50 aa are shown in a schematic diagram.

ICAP-1α is a novel serine/threonine-rich 20-kD protein. (A) Sequence of ICAP-1. The translated amino acid sequence of ICAP-1α is shown. 50 amino acids absent in ICAP-1β are indicated in underlined italics. The nucleotide sequence data are available from GenBank/EMBL/DDBJ under accession number AF012023. (B) Northern blot analysis of ICAP-1α. Northern blots of transcripts from various human tissues (Clontech) were hybridized with ICAP-1α cDNA probe. The 0.9-kb mRNA is expressed in all eight tissue samples. The minor 1.3-kb mRNA, derived from the use of the downstream polyadenylation site, is also expressed in varying amount in all eight samples. (C) Western blot analysis of ICAP-1. ICAP-1α and ICAP-1β were detected in the total cell lysate (5 μg) from 293T cells using polyclonal anti-ICAP-1 antibody (lane 1). The assignments of ICAP-1α and ICAP-1β were confirmed by transiently expressing ICAP-1α (lane 2) and ICAP-1β cDNA (lane 3) in 293T cells. (D) The full-length 200-aa ICAP-1α and an alternatively spliced ICAP-1β lacking internal 50 aa are shown in a schematic diagram.