Table I.
ICAP1: Integrin Cytoplasmic Domain Interaction in Yeast Genetic Screen
| Baits | Sequence* | Interaction‡ | β-gal. Activity§ | |||
|---|---|---|---|---|---|---|
| β1cyto | KLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK | + | ND | |||
| β2cyto | KALIHLSDLREYRRFEKEKLKSQWNN DNPLFKSATTTVMNPKFAES | − | <1.0 | |||
| β1(e16) | GENPIYKSAVTTVVNPKYEGK | + | 149.5 ± 18.3 | |||
| β2(e16) | DNPLFKSATTTVMNPKFAES | − | <1.0 | |||
| β3(e16) | ANNPLYKEATSTFTNITYRGT | − | <1.0 | |||
| β5(e16) | ASNPLYRKPISTHTVDFTFNKSYNGTVD | − | 2.41 ± 3.0 | |||
| αL | KVGFFKRNLKEKMEAGRGVPNGIPAEDSEQLASGQEAGDPGCLKPLHEKDSESGGGD | − | <1.0 | |||
| α2 | KVGFFKRKYEKMTKNPDEIDETTELSS | − | ND |
*
The conserved NPXY motifs of integrin β chains are indicated in bold letters. β1 cyto, complete cytoplasmic domain of integrin β1. β1(E16), COOH-terminal 21 aa of integrin β1. αL, complete cytoplasmic domain of integrin αL.
‡
Positive interaction denotes growth of blue colonies on X-gal indicator plates lacking leucine.
§
β-galactosidase activity was derived from a minimum four independent measurements.