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. 1997 Sep 8;138(5):1041–1053. doi: 10.1083/jcb.138.5.1041

Figure 2.

Figure 2

Figure 2

Figure 2

Binucleate cell production and growth properties of motor mutants. (A) dyn1Δ kip3Δ cells form binucleate cells at 26°C. The arrowhead points to a cell with an aberrant nuclear morphology. (B) Quantitation of binucleate cells produced at 26 and 12°C. For A and B, cells of the indicated genotypes were grown in rich media to log phase at 26°C and stained with DAPI or shifted to 12°C for 24 h before staining. Binucleate cells are those in which two or more nuclear DNA masses could be visualized in one cell body. Each value represents the average of three experiments in which 200 cells were examined. Error bars indicate the SEM. (C) Cells of the indicated genotypes were spotted onto solid media and incubated at the temperature noted. Two spots for each combination are shown, with the second equal to a 40-fold dilution of the first. Yeast strains used: wild-type (MAY589), kip3Δ (MAY4517), dyn1Δ (MAY4434), kip2Δ (MAY2038), dyn1Δ kip3Δ (MAY4544), kip2Δ kip3Δ (MAY4716), dyn1Δ kip2Δ (MAY4566), and dyn1Δ kip2Δ kip3Δ (MAY4560).