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. 1997 Sep 8;138(5):1055–1066. doi: 10.1083/jcb.138.5.1055

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The dynein-specific 70.1 antibody causes a reduction in the efficiency with which cytoplasmic dynein associates with the mitotic spindle in vivo. Monkey CV-1 cells in metaphase with bipolar mitotic spindles were selected by phase contrast microscopy and microinjected with either a control antibody (A) or the dynein-specific 70.1 monoclonal antibody (B). The cells were then fixed and processed for immunofluorescence microscopy using the DNA-specific dye DAPI and the 74.1 antibody, which is specific for the light intermediate chain of cytoplasmic dynein as indicated. Bar, 10 μm.