Table I.

Effect of KHC fragments on Cell Membrane Resealing in Activated Eggs of L. pictus

		Percent resealing (n)
Reagents injected		10–30 min		31–60 min		61–120 min
Stalk-tail		100 (13)		0 (17)		0 (9)
Stalk		100 (13)		100 (17)		100 (11)

Embryonic cells were injected with fragments of KHC and were then wounded by micropipette penetration at different times after injection. Wounding and resealing were monitored both by Fura-2 fluorescence and by visual inspection (Steinhardt et al., 1994; Bi et al., 1995). Stalk-tail injection completely inhibited resealing after a delay of 30 min. Injection of stalk fragment had no effect on resealing. n is the total number of cells tested (same for Table II).