Table II.
Effect of BDM on Cell Membrane Resealing in Activated Eggs of L. pictus
| Concentration of BDM | Percent resealing (n) | |||||||
|---|---|---|---|---|---|---|---|---|
| 1–20 min | 21–40 min | 41–60 min | Wash | |||||
| 20 | 100 (15) | 100 (22) | 100 (28) | – | ||||
| 40 | 100 (25) | 59 (22) | 0 (17) | – | ||||
| 50 | 74 (27) | 10 (30) | 0 (42) | 100 (16) | ||||
Cells were treated with BDM and then wounded to test for resealing at different times. At high concentration of BDM (50 mM), cells showed failure to reseal as early as 8 min after BDM addition. The inhibition of resealing was completely reversed after BDM was washed away. (Cells were tested 15–45 min after BDM washout.) In parallel experiments, resealing in unfertilized sea urchin eggs was not affected by 50 mM BDM (100% normal resealing 21–70 minutes after BDM addition, n = 15).