Table II.
Quantitative Mating Defect of fps1Δ Mutants
| a strain | α strain | Mating efficiency | Prezygotes | |||
|---|---|---|---|---|---|---|
| % | % | |||||
| 1. WT (IH3196) | WT (IH3190) | 100 | 0.8 ± 0.2 | |||
| 2. fps1Δ (JP147) | WT (IH3190) | 46 ± 10 | 61 ± 17 | |||
| 3. WT (IH3196) | fps1Δ (JP226) | 39 ± 8 | 32 ± 5 | |||
| 4. fps1Δ (JP147) | fps1Δ (JP226) | 32 ± 15 | 18 ± 9 | |||
| 5. WT (IH3196) | WT (IH2350) | 100 | ND | |||
| 6. WT (IH3196) | fus1 fus2 (IH2351) | 35 ± 10 | ND | |||
| 7. fps1Δ (JP147) | fus1 fus2 (IH2351) | 1.4 ± 1.0 | ND |
Mating efficiencies were calculated as described in Material and Methods. Mating efficiencies were normalized to the isogenic wild-type mating. For lines 2–4, the values were normalized to the efficiency of IH3196 × IH3190 mating (line 1). Values are the means of at least four experiments ± SD. For lines 6 and 7, the values were normalized to the efficiency of IH3196 × IH2350 mating (line 5) and are the mean of three experiments ± SD. Percentage of prezygotes represents the number of prezygotes/(zygotes + prezygotes). At least 100 partnered cells were counted for each experiment. Values are the means of three experiments ± SD.