Table IV.

Effect of Altering GPD1 Expression on the Defect in Cell Fusion of fps1Δ Mutants

		Percentage of prezygotes in matings to *
Genotype		α GPD1				α gpd1Δ
a WT		0.75 ± 0.55				0.75 ± 0.55
a gpd1Δ		1.50 ± 0.75				1.75 ± 0.55
a fps1Δ		42.3 ± 6.7				55.5 ± 4.6
a fps1Δ gpd1Δ		14.0 ± 2.5				26.0 ± 7.2
		α WT + vector				α WT + 2μ GPD1
a WT + vector		2				<1
a WT + 2μ GPD1		5				<1
a fps1Δ + vector		20				7
a fps1Δ + 2μ GPD1		84				33

In the top four lines, α strains were WT (IH3186) and gpd1Δ (JP236). a strains were WT (IH3196), gpd1Δ (JP168), fps1Δ (JP147), and fps1Δ gpd1Δ (JP165). At least 100 partnered cells were counted for each experiment. Values are the means of four experiments ± SD. In the bottom four lines the α strain was IH3190. a strains were WT + vector (JP400), WT + YEpGPD1 (JP199), fps1Δ + vector (JP401), and fps1Δ + YEpGPD1 (JP200). More than 100 partnered cells were counted per sample. Data are from one representative experiment.  

^* Percentage of prezygotes represents the number of prezygotes/(zygotes + prezygotes).