Table VI.

The PKC1-R398P Allele Causes a Defect in Cell Fusion

Genotype		Plasmid		Percentage of prezygotes*		Percentage of partnership
a WT		vector		2.0 ± 0.7		41.6 ± 1.6
a WT		PKC1-R398P		16.3 ± 1.1		46.3 ± 3.9
a fps1Δ		vector		26.3 ± 6.4		40.0 ±2.5
a fps1Δ		PKC1-R398P		82.6 ± 4.9		44.0 ± 0.7
a gpd1Δ		vector		2.0 ± 0.7		40.7 ± 7.9
a gpd1Δ		PKC1-R398P		18.3 ± 1.5		41.0 ± 1.9

Percentage of partnership represents the number of (zygotes + prezygotes)/total cells. a strains were WT (IH3196), fps1Δ (JP163), and gpd1Δ (JP168). The strains were transformed with either YCp50 containing PKC1-R398P (pJP67) or YCp50 alone and were mated to a wild-type α strain (IH3186). More than 100 partnered cells were counted per experiment to determine the percentage of prezygotes. More than 200 total cells were counted per experiment to determined the percentage of partnership. Values are the mean of three experiments ± SD.  

^* Percentage of prezygotes represents the number of prezygotes/(zygotes + prezygotes).