Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Sep 8;138(5):1105–1116. doi: 10.1083/jcb.138.5.1105

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Cdc25B was lost by treatment with cycloheximide. A series of cultures of tsBN2 cells synchronized in S phase with HU was incubated at 40°C in the absence (nontreated) or presence (CHX-treated) of cycloheximide (10 μg/ml). Every hour, cells were harvested to prepare the total cell extracts. (A) p34^cdc2/cyclin B complexes of total cell extracts prepared from nontreated (–•–) or cycloheximide-treated cells (–○–) were immunoprecipitated using the anti-cyclin B antibody and assayed for histone H1 kinase activities. (B) Proteins of total cell extracts prepared from nontreated (−CHX) and CHX-treated (+CHX) cells were electrophoresed in SDS-10% polyacrylamide gel and analyzed by immunoblotting using as a probe the antibodies against the proteins indicated.

Cdc25B was lost by treatment with cycloheximide. A series of cultures of tsBN2 cells synchronized in S phase with HU was incubated at 40°C in the absence (nontreated) or presence (CHX-treated) of cycloheximide (10 μg/ml). Every hour, cells were harvested to prepare the total cell extracts. (A) p34^cdc2/cyclin B complexes of total cell extracts prepared from nontreated (–•–) or cycloheximide-treated cells (–○–) were immunoprecipitated using the anti-cyclin B antibody and assayed for histone H1 kinase activities. (B) Proteins of total cell extracts prepared from nontreated (−CHX) and CHX-treated (+CHX) cells were electrophoresed in SDS-10% polyacrylamide gel and analyzed by immunoblotting using as a probe the antibodies against the proteins indicated.