Figure 4.
Microsomal processing of preprominiPLAP. miniPLAP RNA was added in cotranslational assays with [35S]M and RM. (A) Products deriving from processing of preprominiPLAP by K562.ATCC (lanes 1–4) and .48 RM (lanes 5–8), treated with buffer alone (lanes 1 and 5), buffer and PI-PLC (lanes 2 and 6), alkaline/hydroxylamine alone (lanes 3 and 7), or alkaline/hydroxylamine and PI-PLC (lanes 4 and 8). (B) Products deriving from HeLa cell RM were treated with buffer or hydroxylamine and PI-PLC and then partitioned in TX-114 detergent. A, aqueous phase; D, detergent phase. (C and D) Cotranslational processing of preprominiPLAP in the presence of HeLa RM was stopped after the indicated times, the mixture treated with buffer (lanes 1–6) or PI-PLC (lanes 7–12) and the products analyzed. (D) Densitometric data.