Table 1.
ND-PAGE analyses of the GPI anchors in DAF, PLAP, and CD59 from KAPLAP and K48PLAP transfectants
| Cell type | Unmodified inositol | Acylated inositol |
|---|---|---|
| Placenta | ||
| PLAP protein | 91.0 | 9.0 |
| K48PLAP | ||
| DAF protein | <5 | >95 |
| PLAP protein | <5 | >95 |
| PLAP activity | <5 | >95 |
| KAPLAPa | ||
| DAF protein | 60.3 ± 4.1* | 39.7 ± 4.1 |
| PLAP protein | 30.1 ± 1.6 | 69.9 ± 1.6 |
| PLAP activity† | 29.7 ± 3.8 | 70.3 ± 3.8 |
| CD59 protein | 59.8 ± 16 | 40.2 ± 16 |
| KAPLAPb | ||
| DAF protein | 49.2 | 50.8 |
| PLAP protein | 36.7 | 63.3 |
*
The proportions of hydrophilic and detergent-bound DAF and PLAP in each lane were determined by densitometry of autoradiographs or stained gels. Values represent the mean and SD of two separate analyses.
†
Gels were stained for alkaline phosphatase activity with an alkaline phosphatase assay kit (Sigma). Bands were visualized using an indigogenic dye substrate. Values represent the mean and SD of three analyses.