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Analysis of vWf multimers in plasma. Human plasma (Hu) or mouse plasma from wild-type (+/+), heterozygous (±), or homozygous mutant (−/−) mice was layered on an SDS-1% agarose gel. vWf multimers were separated by discontinuous SDS/agarose gel electrophoresis and detected by incubation with 125I-labeled polyclonal anti-human vWf antibody. Autoradiograph of the gel is shown.
