Figure 5.

Platelet-vessel wall interactions after ferric chloride-induced injury. Wild-type (striated columns, n = 15) or vWf-deficient mice (black columns, n = 12) were injected with fluorescently labeled platelets of matching genotype, and their mesenteries were exposed. Arterioles (60–100 μm in diameter) were selected, and a vascular injury was provoked by superfusion with ferric chloride. Any platelet interaction with the vessel wall, either brief or stable, was counted. The presence of platelet thrombi also was recorded. For each vessel studied, the total number of interactive platelets during the first 2 min, 5 min, or during the entire 10 min of filming was recorded and was classified into categories, as indicated on the left.