Table 1.
Hematological and coagulation analysis of vWf-deficient mice
| +/+ | +/− | −/− | |
|---|---|---|---|
| vWf antigen, % | 96 ± 5.6 | 49 ± 3.6* | Not detectable |
| FVIII activity, % | 139.5 ± 7.5 | 81 ± 4* | 27.6 ± 1.1* |
| PT, sec | 14.7 ± 0.42 | 14.8 ± 0.63 | 14.9 ± 0.67 |
| aPTT, sec | 23.2 ± 0.5 | 24.4 ± 0.4 | 34.5 ± 1* |
| Bleeding time, sec | 69.7 ± 5.2 | 91.9 ± 11.2 | 499 ± 33.4* |
| Platelets, ×109/l | 837 ± 43 | 986 ± 86 | 931 ± 27 |
| RBC, ×1012/l | 7.88 ± 0.12 | 7.67 ± 0.21 | 7.72 ± 0.17 |
| WBC, ×109/l | 2.8 ± 0.33 | 3.37 ± 0.36 | 3.07 ± 0.34 |
| Hematocrit, % | 37.1 ± 0.6 | 35.4 ± 1.1 | 36.9 ± 1 |
| Hemoglobin, g/dl | 12.8 ± 0.24 | 12.4 ± 0.22 | 12.7 ± 0.31 |
n = 8–13 mice except for the bleeding time, where n = 15–21. All mice were 2–3 months old. For the ELISA and FVIII activity data, 100% was defined as being the percentage of antigen or activity found in a pool of plasma from 10 C57BL/6J mice. Our mice were on a mixed C57BL/6J/129Sv background, a difference that might account for the higher activity found in our mice compared with the pool.
*
P < 0.0001 when compared with wild type.