Table 1.
Effects of enzyme inhibitors on THF-diol or E2 stimulation of MCF-7 cell proliferation at 24 hr.
| Enzyme/inhibitor | Treatment | Cells/well × 10−4 (± SE) |
|---|---|---|
| Phospholipase A2 | EtOH | 7.63 ± 0.89 |
| Quinacrine (Quin) | Quinacrine (2.5 μM) | 2.00 ± 0.27a |
| THF-diol (24 μM) | 35.75 ± 2.15b | |
| E2 (5 nM) | 43.75 ± 1.41b | |
| THF + Quin | 6.50 ± 0.46 | |
| E2 + Quin | 10.38 ± 0.92 | |
| Phospholipase A2 | EtOH | 9.63 ± 0.65 |
| Quinacrine (Quin) | Quinacrine (2.5 μM) | 1.63 ± 0.38c |
| Linoleic acid (36 μM) | 13.13 ± 0.92 | |
| Quin + linoleic acid | 11.63 ± 0.625 | |
| COX-1 and COX-2 | EtOH | 7.38 ± 0.75 |
| Indomethacin (Indo) | Indomethacin (50 μM) | 6.25 ± 0.65 |
| THF-Diol (24 μM) | 57.75 ± 1.89d | |
| E2 5 (nM) | 76.38 ± 2.9d | |
| Indo +THF-diol | 62.00 ± 2.73d | |
| Indo + E2 | 71.12 ± 2.89d | |
| LOX-5 | EtOH | 6.25 ± 0.45 |
| REV-5901 (Rev) | REV-5901 (10 μM) | 6.25 ± 0.49 |
| THF-Diol (24 μM) | 68.75 ± 2.67e | |
| E2 (5 nM) | 83.13 ± 5.19e | |
| REV + THF-Diol | 41.25 ± 1.7e,f | |
| REV + E2 | 62.88 ± 4.02e,f | |
| LOX-12 | EtOH | 6.5 ± 0.53 |
| Baicalein (Baic) | Baicalein (5 μM) | 5.63 ± 0.53 |
| THF-Diol (24 μM) | 59.00 ± 2.82g | |
| 5 nM E2 | 63.38 ± 1.95g | |
| Baic + THF-diol | 47.2 ± 1.7g,h | |
| Baic + E2 | 55.88 ± 1.69g,i | |
| LOX-5 and LOX-12 | EtOH | 6.875 ± 0.79 |
| NDGA | NDGA (25 μM) | 8.25 ± 0.96 |
| THF-diol (24 μM) | 49.25 ± 1.37j | |
| E2 (5 nM) | 59.625 ± 4.24j | |
| THF + NDGA | 10.375 ± 0.63 | |
| E2 + NDGA | 15.0 ± 0.89 | |
| Aromatase | EtOH | 7.25 ± 0.90 |
| Formestane (Form) | Formestane (50 nM) | 8.5 ± 1.05 |
| THF-diol 24 μM) | 65.25 ± 3.94k | |
| E2 (5 nM) | 68.88 ± 3.94k | |
| Form + THF-diol | 53.12 ± 3.19k | |
| Form + E2 | 64.75 ± 1.95k |
Significant difference from EtOH group (p < 0.05).
Significant difference from all other groups (p < 0.001).
Significant difference from all other groups (p < 0.01).
Significant difference from EtOH and indomethacin groups (p < 0.001).
Significant difference from EtOH or REV-5901 groups (p < 0.001).
Significant difference from E2 or THF-Diol groups (p < 0.001).
Significant difference from EtOH group (p < 0.001).
Significant difference from THF-Diol group (p < 0.001).
Significant difference from E2 group (p < 0.05).
Significant difference from EtOH, NDGA, THF + NDGA and E2 + NDGA groups (p < 0.001).
Significant difference from EtOH and formestane groups (p < 0.001).