Table 2.
Triacylglycerol molecular species and mass content in lipid extracts of mouse cervical dorsal root ganglia at different agesa
| [M + Li]+ | Major species | 2 months | 5 months | 8 months |
|---|---|---|---|---|
| 783.7 | 14 : 0/16 : 0/16 : 1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 |
| 809.7 | 14 : 0/16 : 0/18 : 2, 16 : 0/16 : 1/16 : 1 | 0.9 ± 0.1 | 1.1 ± 0.2 | 1.0 ± 0.1 |
| 811.7 | 14 : 0/16 : 0/18 : 1, 16 : 0/16 : 0/16 : 1 | 1.9 ± 0.1 | 1.6 ± 0.2 | 1.6 ± 0.2 |
| 813.7 | T16 : 0 | 0.8 ± 0.1 | 0.6 ± 0.1 | 0.8 ± 0.2 |
| 833.7 | 16 : 1/16 : 1/18 : 2 | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.4 ± 0.1 |
| 835.7 | 14 : 0/18 : 1/18 : 2, 16 : 0/16 : 1/18 : 2, 16 : 1/16 : 1/18 : 1 | 2.8 ± 0.2 | 2.8 ± 0.3 | 1.9 ± 0.1 |
| 837.7 | 14 : 0/18 : 0/18 : 2, 16 : 0/16 : 0/18 : 2, 16 : 0/16 : 1/18 : 1 | 4.9 ± 0.1 | 5.2 ± 0.4 | 4.5 ± 0.3 |
| 839.7 | 16 : 0/16 : 0/18 : 1, 16 : 0/16 : 1/18 : 0 | 4.6 ± 0.3 | 3.2 ± 0.2 | 3.0 ± 0.3 |
| 853.7 | 16 : 0/17 : 0/18 : 1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 859.8 | 16 : 0/16 : 1/20 : 4, 16 : 1/18 : 2/18 : 2 | 0.7 ± 0.1 | 0.9 ± 0.1 | 0.6 ± 0.1 |
| 861.8 | 16 : 0/18 : 2/18 : 2, 16 : 1/18 : 1/18 : 2 | 3.6 ± 0.2 | 3.5 ± 0.3 | 2.7 ± 0.2 |
| 863.8 | 16 : 0/18 : 1/18 : 2, 16 : 1/18 : 0/18 : 2, 16 : 1/18 : 1/18 : 1 | 6.8 ± 0.4 | 7.2 ± 0.6 | 5.0 ± 0.3 |
| 865.8 | 16 : 0/18 : 0/18 : 2, 16 : 0/18 : 1/18 : 1 | 6.4 ± 0.5 | 6.0 ± 0.4 | 4.2 ± 0.4 |
| 867.8 | 16 : 0/18 : 0/18 : 1 | 1.0 ± 0.3 | 0.7 ± 0.1 | 0.7 ± 0.1 |
| 873.8 | 17 : 1/18 : 2/18 : 2 | 0.6 ± 0.1 | 0.4 ± 0.1 | 0.5 ± 0.1 |
| 885.8 | 16 : 0/18 : 2/20 : 4, T18 : 2 | 0.8 ± 0.1 | 0.8 ± 0.1 | 0.7 ± 0.1 |
| 887.8 | 16 : 0/18 : 1/20 : 4, 18 : 0/18 : 2/18 : 3, 18 : 1/18 : 2/18 : 2 | 2.5 ± 0.1 | 2.5 ± 0.2 | 2.0 ± 0.2 |
| 889.8 | 18 : 0/18 : 2/18 : 2, 18 : 1/18 : 1/18 : 2 | 4.6 ± 0.2 | 4.2 ± 0.4 | 3.1 ± 0.2 |
| 891.8 | 16 : 0/18 : 2/20 : 1, 18 : 0/18 : 1/18 : 2, T18 : 1 | 4.7 ± 0.3 | 4.6 ± 0.4 | 2.2 ± 0.2 |
| 893.8 | 16 : 0/18 : 1/20 : 1, 18 : 0/18 : 1/18 : 1 | 1.5 ± 0.2 | 0.9 ± 0.1 | 0.6 ± 0.1 |
| 911.8 | 16 : 0/18 : 1/22 : 6 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 915.8 | 18 : 0/18 : 1/20 : 4 | 0.4 ± 0.1 | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 919.8 | 18 : 1/18 : 1/20 : 1 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.2 ± 0.1 |
| Total TAGb | 55.5 ± 2.4 | 49.9 ± 5.3* | 41.7 ± 3.0** | |
| 16 : 0 FA | 46.3 ± 1.9 (27.8 ± 1.2) | 37.3 ± 5.5 (24.9 ± 3.7) | 35.6 ± 4.3 (28.5 ± 3.5) | |
| 18 : 0 FA | 15.0 ± 2.2 (9.0 ± 1.3) | 12.3 ± 0.6 (8.2 ± 0.4) | 8.2 ± 1.9 (6.5 ± 1.9) | |
| 18 : 1 FA | 50.4 ± 3.2 (30.3 ± 1.9) | 49.2 ± 4.2 (32.9 ± 2.8) | 35.8 ± 1.4 (28.6 ± 1.1) | |
| 18 : 2 FA | 20.8 ± 1.6 (12.5 ± 0.9) | 20.7 ± 2.8 (13.8 ± 1.9) | 18.8 ± 2.0 (15.0 ± 1.6) | |
| Odd numbered FA | 9.4 ± 0.2 (5.7 ± 0.2) | 8.5 ± 0.8 (5.7 ± 0.6) | 7.3 ± 0.7 (5.8 ± 0.6) | |
| Polyunsaturated FAc | 31.3 ± 1.5 (18.8 ± 1.0) | 29.4 ± 3.1 (19.6 ± 2.1) | 25.8 ± 1.5 (20.6 ± 1.2) | |
| FA w/20 or 22 carbons | 7.8 ± 0.7 (4.7 ± 0.4) | 6.8 ± 0.6 (4.6 ± 0.4) | 5.4 ± 0.7 (4.3 ± 0.5) |
Mouse cervical DRG lipids were extracted by a modified Bligh and Dyer procedure and the TAG molecular species in the lipid extracts were identified and quantified using a 2D ESI mass spectrometric technique as described in the Materials and methods. The results are expressed in nmol/mg of protein and represent X ± SEM of at least four different animals, whereas the data in parentheses are the percentages of the acyl chains in TAG molecular species. FA: fatty acyl chain. Only the TAG molecular species that contribute over 0.5 mol% of total DRG TAG in mice at 2 months of age are listed in the Table.
p < 0.05 and
p < 0.05 and
p < 0.001 relative to values at 2 months.
Acyl chains in TAG molecular species containing two or more double bonds.