Table 3.
Triacylglycerol molecular species and mass content in lipid extracts of cervical dorsal root ganglia of control and diabetic micea
| [M + Li]+ | Major species | Control | Diabetes |
|---|---|---|---|
| 783.7 | 14 : 0/16 : 0/16 : 1 | 0.3 ± 0.1 | 0.1 ± 0.1 |
| 809.7 | 14 : 0/16 : 0/18 : 2, 16 : 0/16 : 1/16 : 1 | 1.1 ± 0.2 | 0.2 ± 0.1 |
| 811.7 | 14 : 0/16 : 0/18 : 1, 16 : 0/16 : 0/16 : 1 | 1.6 ± 0.2 | 0.4 ± 0.1 |
| 813.7 | T16 : 0 | 0.62 ± 0.08 | 0.25 ± 0.03 |
| 833.7 | 16 : 1/16 : 1/18 : 2 | 0.48 ± 0.06 | 0.18 ± 0.02 |
| 835.7 | 14 : 0/18 : 1/18 : 2, 16 : 0/16 : 1/18 : 2, 16 : 1/16 : 1/18 : 1 | 2.8 ± 0.3 | 0.8 ± 0.1 |
| 837.7 | 14 : 0/18 : 0/18 : 2, 16 : 0/16 : 0/18 : 2, 16 : 0/16 : 1/18 : 1 | 5.2 ± 0.4 | 1.9 ± 0.2 |
| 839.7 | 16 : 0/16 : 0/18 : 1, 16 : 0/16 : 1/18 : 0 | 3.2 ± 0.2 | 1.1 ± 0.1 |
| 853.7 | 16 : 0/17 : 0/18 : 1 | 0.31 ± 0.06 | 0.05 ± 0.01 |
| 859.8 | 16 : 0/16 : 1/20 : 4, 16 : 1/18 : 2/18 : 2 | 0.9 ± 0.1 | 0.3 ± 0.1 |
| 861.8 | 16 : 0/18 : 2/18 : 2, 16 : 1/18 : 1/18 : 2 | 3.5 ± 0.3 | 1.7 ± 0.1 |
| 863.8 | 16 : 0/18 : 1/18 : 2, 16 : 1/18 : 0/18 : 2, 16 : 1/18 : 1/18 : 1 | 7.2 ± 0.6 | 2.6 ± 0.3 |
| 865.8 | 16 : 0/18 : 0/18 : 2, 16 : 0/18 : 1/18 : 1 | 6.0 ± 0.4 | 1.7 ± 0.2 |
| 867.8 | 16 : 0/18 : 0/18 : 1 | 0.54 ± 0.06 | 0.32 ± 0.03 |
| 873.8 | 17 : 1/18 : 2/18 : 2 | 0.35 ± 0.05 | 0.15 ± 0.02 |
| 885.8 | 16 : 0/18 : 2/20 : 4, T18 : 2 | 0.8 ± 0.1 | 0.5 ± 0.1 |
| 887.8 | 16 : 0/18 : 1/20 : 4, 18 : 0/18 : 2/18 : 3, 18 : 1/18 : 2/18 : 2 | 2.5 ± 0.2 | 1.2 ± 0.1 |
| 889.8 | 18 : 0/18 : 2/18 : 2, 18 : 1/18 : 1/18 : 2 | 4.2 ± 0.4 | 2.3 ± 0.2 |
| 891.8 | 16 : 0/18 : 2/20 : 1, 18 : 0/18 : 1/18 : 2, T18 : 1 | 4.6 ± 0.4 | 1.8 ± 0.2 |
| 893.8 | 16 : 0/18 : 1/20 : 1, 18 : 0/18 : 1/18 : 1 | 0.9 ± 0.1 | 0.4 ± 0.1 |
| 911.8 | 16 : 0/18 : 1/22 : 6 | 0.30 ± 0.03 | 0.16 ± 0.02 |
| 915.8 | 18 : 0/18 : 1/20 : 4 | 0.31 ± 0.05 | 0.18 ± 0.02 |
| 919.8 | 18 : 1/18 : 1/20 : 1 | 0.29 ± 0.03 | 0.09 ± 0.01 |
| Total TAGb | 49.9 ± 5.3 | 22.0 ± 4.7** | |
| 16 : 0 FA | 37.3 ± 5.5 (24.9 ± 3.7) | 15.6 ± 3.5 (23.9 ± 2.5) | |
| 18 : 0 FA | 12.3 ± 0.6 (8.2 ± 0.4) | 7.1 ± 1.4 (11.1 ± 1.2) | |
| 18 : 1 FA | 49.2 ± 4.2 (32.9 ± 2.9) | 19.4 ± 4.3 (29.1 ± 1.0) | |
| 18 : 2 FA | 20.7 ± 2.8 (13.8 ± 1.9) | 15.9 ± 3.6 (24.2 ± 2.5) | |
| Odd-numbered FA | 8.5 ± 0.8 (5.7 ± 0.6) | 2.2 ± 0.8 (3.7 ± 1.4) | |
| Polyunsaturated FAc | 29.4 ± 3.1 (19.6 ± 2.1) | 17.4 ± 4.4 (26.6 ± 3.1) | |
| FA w/20 or 22 carbons | 6.8 ± 0.6 (4.6 ± 0.4) | 1.5 ± 0.5 (2.2 ± 0.4) |
Mouse diabetes was induced by streptozotocin treatment. Lipids of mouse cervical dorsal root ganglia (DRG) were extracted using a modified Bligh and Dyer procedure and the TAG molecular species in the lipid extracts were identified and quantified by the 2D ESI mass spectrometric approach after intrasource separation as described in the Materials and methods. The results are expressed in nmol/mg of protein and represent X ± SEM of a minimum of four different animals. Only the TAG molecular species that contribute over 0.5 mol% of total DRG TAG in control mice are listed in the Table.
p < 0.001 relative to values in controls.
p < 0.001 relative to values in controls.
Acyl chains in TAG molecular species containing two or more double bonds.