Fig. 2.

Representative 2D electrospray ionization/mass spectrometry (ESI/MS) analyses of anionic lipids and neutral lipid chlorine-adducts in a lipid extract of mouse dorsal root ganglia. The diluted lipid extract from a wild-type mouse at 4 months of age used for acquisition of spectrum (a) in Fig. 1 was employed. Each MS or MS/MS trace of the 2D ESI mass spectrum was acquired by sequentially programmed customized scans operating utilizing Xcalibur software. ‘PI’ denotes precursor-ion scanning, ‘NL’ stands for neutral loss scanning, and ‘IS’ represents internal standard. All mass spectral traces are displayed after normalization to the most intense peak (base peak) in each individual trace.