Fig. 6.

Two-dimensional electrospray ionization mass/spectrometry fingerprint and quantitation of triacylglycerol (TG) molecular species in the dorsal root ganglia lipid extracts of wild-type and apoE knockout mice. Dorsal root ganglia lipids of wild-type (Panel a) and apolipoprotein E knockout (Panel b) mice at 4 months of age were extracted by a modified Bligh and Dyer method. Two-dimensional electrospray ionization/mass spectrometry analyses were performed by neutral loss scanning of all naturally occurring fatty acids (building blocks of TG molecular species) in the positive-ion mode in the presence of LiOH as described under Materials and methods.