Table 1.
Mass contents of sphingolipid molecular species in the dorsal root ganglia of wild-type and apolipoprotein E knockout mice at 4 months of agea
| |
|
Molecular species |
WT mice |
ApoE KO mice |
|---|---|---|---|---|
| [M - H]− | Sulfatide | |||
| 804.7 | N18:1 sulfatide | 0.3 ± 0.1 | 0.4 ± 0.1 | |
| 806.7 | N18:0 sulfatide | 2.3 ± 0.3 | 3.3 ± 0.1 | |
| 820.7 | OH N18:1 sulfatide | 0.9 ± 0.3 | 1.1 ± 0.3 | |
| 822.7 | OH N18:0 sulfatide | 1.9 ± 0.2 | 2.4 ± 0.3 | |
| 832.7 | N20:1 sulfatide | 0.5 ± 0.1 | 0.6 ± 0.2 | |
| 834.7 | N20:0 sulfatide | 1.6 ± 0.1 | 2.4 ± 0.1 | |
| 848.7 | OH N20:1 sulfatide | 0.5 ± 0.1 | 0.6 ± 0.1 | |
| 850.7 | OH N20:0 sulfatide | 1.1 ± 0.1 | 1.4 ± 0.1 | |
| 860.7 | N22:1 sulfatide | 1.3 ± 0.1 | 1.8 ± 0.2 | |
| 862.7 | N22:0 sulfatide | 4.6 ± 0.4 | 6.6 ± 0.5 | |
| 876.7 | OH N22:1 sulfatide | 1.7 ± 0.2 | 2.5 ± 0.2 | |
| 878.7 | OH N22:0 sulfatide | 3.0 ± 0.2 | 3.8 ± 0.2 | |
| 886.8 | N24:2 sulfatide | 4.4 ± 0.3 | 5.8 ± 0.1 | |
| 888.8 | N24:1 sulfatide | 17.8 ± 1.2 | 25.7 ± 0.6 | |
| 890.8 | N24:0 sulfatide | 13.1 ± 1.1 | 23.6 ± 0.6 | |
| 902.8 | OH N24:2 sulfatide | 0.9 ± 0.1 | 1.0 ± 0.1 | |
| 904.8 | OH N24:1 sulfatide | 1.6 ± 0.2 | 2.0 ± 0.1 | |
| 906.8 | OH N24:0 sulfatide | 3.3 ± 0.3 | 4.7 ± 0.2 | |
| 916.8 | N26:1 sulfatide | 0.7 ± 0.1 | 0.7 ± 0.1 | |
| 918.8 | N26:0 sulfatide | 0.5 ± 0.1 | 0.4 ± 0.1 | |
| 932.8 | OH N26:1 sulfatide | 0.3 ± 0.1 | 0.4 ± 0.1 | |
| 934.8 | OH N26:0 sulfatide | 0.5 ± 0.1 | 0.6 ± 0.1 | |
| Total sulfatide | 62.8 ± 3.1 | 91.4 ± 1.5** | ||
| Hydroxy sulfatide | 15.8 ± 1.0 | 20.3 ± 0.7** |
| [M + CI]− | [M + Li]+ | Galactosylceramide (GalCer) | ||
|---|---|---|---|---|
| 732.6 | 704.6 | N16:1 GalCer | DA | DA |
| 734.6 | 706.6 | N16:0 GalCer | DA | DA |
| 760.6 | 732.6 | N18:1 GalCer | 0.3 ± 0.1 | 0.3 ± 0.1 |
| 762.6 | 734.6 | N18:0 GalCer | 4.0 ± 0.2 | 4.3 ± 0.2 |
| 776.6 | 748.6 | OH N18:1 GalCer | 0.5 ± 0.1 | 0.9 ± 0.2 |
| 778.6 | 750.6 | OH N18:0 GalCer | 3.2 ± 0.2 | 3.6 ± 0.3 |
| 788.6 | 760.6 | N20:1 GalCer | DA | DA |
| 790.6 | 762.6 | N20:0 GalCer | 7.2 ± 0.8 | 7.9 ± 0.5 |
| 804.6 | 776.6 | N21:0/OH N20:1 GalCer | 1.6 ± 0.2 | 1.7 ± 0.3 |
| 806.6 | 778.6 | OH N20:0 GalCer | 8.7 ± 0.7 | 9.7 ± 0.4 |
| 816.6 | 788.6 | N22:1 GalCer | 0.4 ± 0.1 | 0.9 ± 0.1 |
| 818.7 | 790.7 | N22:0 GalCer | 5.3 ± 0.6 | 7.1 ± 0.1 |
| 820.7 | 792.7 | OH N21:0 GalCer | 3.5 ± 0.3 | 3.7 ± 0.5 |
| 832.7 | 804.7 | N23:0/OH N22:1 GalCer | 7.0 ± 0.5 | 7.2 ± 0.4 |
| 834.7 | 806.7 | OH N22:0 GalCer | 19.0 ± 1.1 | 23.7 ± 1.7 |
| 842.7 | 814.7 | N24:2 GalCer | 2.6 ± 0.2 | 2.9 ± 0.2 |
| 844.7 | 816.7 | N24:1 GalCer | 14.0 ± 1.2 | 17.3 ± 0.4 |
| 846.7 | 818.7 | N24:0 GalCer | 19.8 ± 1.9 | 25.2 ± 0.7 |
| 848.7 | 820.7 | OH N23:0 GalCer | 4.4 ± 0.7 | 5.8 ± 0.6 |
| 858.7 | 830.7 | OH N24:2 GalCer | 4.1 ± 0.5 | 3.8 ± 0.4 |
| 860.8 | 832.8 | N25:0/OH N24:1 GalCer | 11.6 ± 0.6 | 13.2 ± 0.4 |
| 862.8 | 834.8 | OH N24:0 GalCer | 24.3 ± 1.9 | 29.2 ± 1.5 |
| 872.8 | 844.8 | N26:1 GalCer | 0.3 ± 0.1 | 0.4 ± 0.1 |
| 874.8 | 846.8 | N26:0 GalCer | 0.2 ± 0.1 | 0.5 ± 0.1 |
| 876.8 | 848.8 | OH N25:0 GalCer | 1.8 ± 0.2 | 2.8 ± 0.4 |
| 888.8 | 860.8 | OH N26:1 GalCer | 0.7 ± 0.1 | 0.6 ± 0.2 |
| 890.8 | 862.8 | OH N26:0 GalCer | 1.9 ± 0.6 | 1.4 ± 0.3 |
| Total GalCer | 146.8 ± 10.5 | 174.1 ± 5.6* | ||
| Hydroxy GalCer | 92.2 ± 4.7 | 107.3 ± 3.8* |
| [M + Li]+ | Sphingomyelin (SM) | |||
|---|---|---|---|---|
| 707.5 | N16:1 SM | 0.3 ± 0.1 | 0.4 ± 0.1 | |
| 709.5 | N16:0 SM | 3.2 ± 0.4 | 3.8 ± 0.1 | |
| 735.5 | N18:1 SM | 0.4 ± 0.1 | 0.5 ± 0.1 | |
| 737.5 | N18:0 SM | 6.0 ± 0.3 | 6.1 ± 0.3 | |
| 765.6 | N20:0 SM | 3.8 ± 0.4 | 3.3 ± 0.3 | |
| 791.6 | N22:1 SM | 0.5 ± 0.2 | 0.5 ± 0.1 | |
| 793.6 | N22:0 SM | 5.2 ± 0.3 | 5.6 ± 0.3 | |
| 807.6 | N23:0 SM | 1.5 ± 0.2 | 1.2 ± 0.2 | |
| 817.6 | N24:2 SM | 2.1 ± 0.2 | 2.9 ± 0.3 | |
| 819.6 | N24:1 SM | 14.0 ± 0.9 | 14.9 ± 0.6 | |
| 821.6 | N24:0 SM | 7.2 ± 0.3 | 7.4 ± 0.3 | |
| 833.6 | N25:1 SM | 0.3 ± 0.1 | 0.3 ± 0.1 | |
| 835.6 | N25:0 SM | 0.3 ± 0.1 | 0.5 ± 0.1 | |
| Total SM | 44.7 ± 1.6 | 47.4 ± 1.1 |
| [M - H]− | Ceramide (Cer) | |||
|---|---|---|---|---|
| 536.5 | N16:0 Cer | 39 ± 3 | 51 ± 6 | |
| 564.5 | N18:0 Cer | 120 ± 3 | 133 ± 7 | |
| 592.5 | N20:0 Cer | 62 ± 5 | 84 ± 4 | |
| 606.5 | N21:0 Cer | 7 ± 1 | 6 ± 1 | |
| 608.5 | OH N20:0 Cer | 11 ± 1 | 15 ± 1 | |
| 620.5 | N22:0 Cer | 60 ± 3 | 80 ± 2 | |
| 632.5 | N23:1 Cer | 2 ± 1 | 4 ± 2 | |
| 634.5 | N23:0 Cer | 25 ± 4 | 27 ± 3 | |
| 636.5 | OH N22:0 Cer | 25 ± 5 | 29 ± 5 | |
| 644.5 | N24:2 Cer | 21 ± 1 | 30 ± 2 | |
| 646.5 | N24:1Cer | 120 ± 6 | 177 ± 5 | |
| 648.5 | N24:0 Cer | 78 ± 7 | 93 ± 6 | |
| 664.5 | OH N24:0 Cer | 36 ± 7 | 42 ± 3 | |
| Total Cer | 593 ± 26 | 769 ± 27** |
a
Mouse DRG lipids were extracted by a modified Bligh and Dyer procedure and the sphingolipid molecular species in the lipid extracts were identified and quantified using a 2D ESI/MS technique as described under Materials and methods. The results are expressed in nmol/mg of protein (except for ceramide in which data are expressed in pmol/mg of protein) and represent X ± SD of six different animals.
DRG, dorsal root ganglia; WT, wild type; ApoE, apolipoprotein E; KO, knockout; DA, presence of a detectable ion peak; ESI, electrospray ionization; MS, mass spectrometry.
*
p < 0.01.
**
p < 0.001.